Detection of mutations in Salmonella entericagyrA, gyrB, parC and parE genes by denaturing high performance liquid chromatography (DHPLC) using standard HPLC instrumentation

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Denaturing high performance liquid chromatography (DHPLC) used in the detection of germline and somatic mutations.

Denaturing high performance liquid chromatography (DHPLC) has been described recently as a method for screening DNA samples for single nucleotide polymorphisms and inherited mutations. Thirty-eight DNAs, 22 of which were heterozygous for previously characterized rearranged transforming gene (RET) or cystic fibrosis transmembrane conductance regulator gene (CFTR) mutations or polymorphisms, were...

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detection of cyp2c18 m1, and m2 alleles within an iranian population (mazandaran) using denaturing high-performance liquid chromatography (dhplc)

background: genetic polymorphisms of cytochrome p450 in humans are the main cause of differences in the metabolism. the allele and genotype frequencies of cyp2c19 and cyp2c9 have been studied in some iranian populations. the aim of present study was to examine the frequencies of cyp2c18m1, and cyp2c18m2, alleles in the mazandarani ethnic group among iranian population. materials and methods: in...

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2005

ISSN: 1460-2091,0305-7453

DOI: 10.1093/jac/dki293